A novelBacillus nattoplasmid pLS32 capable of replication inBacillus subtilis
نویسندگان
چکیده
منابع مشابه
Characterization of the replication region of plasmid pLS32 from the Natto strain of Bacillus subtilis.
Plasmid pL32 from the Natto strain of Bacillus subtilis belongs to a group of low-copy-number plasmids in gram-positive bacteria that replicate via a theta mechanism of replication. We studied the DNA region encoding the replication protein, RepN, of pLS32, and obtained the following results. Transcription of the repN gene starts 167 nucleotides upstream from the translational start site of rep...
متن کاملFunctional analysis of the stability determinant AlfB of pBET131, a miniplasmid derivative of bacillus subtilis (natto) plasmid pLS32.
Bacillus subtilis plasmid pBET131 is a derivative of pLS32, which was isolated from a natto strain of Bacillus subtilis. The DNA region in pBET131 that confers segregational stability contains an operon consisting of three genes, of which alfA, encoding an actin-like ATPase, and alfB are essential for plasmid stability. In this work, the alfB gene product and its target DNA region were studied ...
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We identified a signaling pathway that prevents initiation of sporulation in Bacillus subtilis when replication initiation is impaired. We isolated mutations that allow a replication initiation mutant (dnaA) to sporulate. These mutations affect a small open reading frame, sda, that was overexpressed in replication initiation mutants and appears to be directly regulated by DnaA. Mutations in rep...
متن کاملUtilization of subsidiary chromosomal replication terminators in Bacillus subtilis.
The Bacillus subtilis merodiploid strain GSY1127 contains a large nontandem duplication of a portion of its chromosome within its left (anticlockwise) replication segment. This causes displacement of the replication terminus region to a noticeably asymmetric location relative to oriC. The utilization of the subsidiary replication terminators, TerIII and TerV, in the merodiploid strain has been ...
متن کاملRegulation of DNA replication and cellular responses to perturbations in replication in the bacterium Bacillus subtilis
When a cell grows and divides to give rise to genetically identical cells, the genome of the cell is duplicated prior to cell-division. The process of genomic duplication is called DNA replication, and is closely coordinated with other processes in the cell, such as growth rate, and cell division. The mechanisms that regulate when DNA replication initiates and how cells respond to perturbations...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1998
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(98)00015-5